After a lower limb injury or surgery, older patients with underlying cardiovascular comorbidities are at a higher risk of injury or re-injury when they walk again. Early mobility and rehabilitation are needed immediately after surgery, and older patients need equipment to reduce the forces on the joints, bones, muscles and ligaments of the lower extremities. Current devices used to unload lower limbs during rehabilitation include walkers, parallel bars, therapist assisted belts, overhead suspension belts, and swimming pools, all of which have disadvantages associated with their use that can interfere with the patient’s recovery, and anti-gravity running tables are newly developed new weight-loss rehabilitation techniques. Before using the antigravity treadmill rehabilitation concept in elderly patients with potential cardiovascular comorbidities, it is necessary to evaluate and study safety in healthy individuals. In all antigravity conditions, stride length increased significantly during running compared to normal walking, and knee and ankle movement patterns were more consistent with other means of weight loss than patterns were with normal walking. The application of antigravity technique does not change capillary perfusion, macrovascular circulation and oxygenation in the head. Applying antigravity to healthy subjects in a stationary upright position and walking on a treadmill reduces load on the lower extremities and maintains a normal gait with minimal risk in terms of head perfusion and blood vessel flow.